p babe sin puro tet cmv rhoa biosensor Search Results


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Santa Cruz Biotechnology rhoc primary antibody
A . Alignment of potential miR-21 binding sites in the 3′UTR of <t>the</t> <t>RhoB</t> mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and <t>RhoC</t> protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).
Rhoc Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cytoskeleton Inc rho activator ii
A . Alignment of potential miR-21 binding sites in the 3′UTR of <t>the</t> <t>RhoB</t> mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and <t>RhoC</t> protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).
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UTECH Products Inc rhoa/rho kinase
A . Alignment of potential miR-21 binding sites in the 3′UTR of <t>the</t> <t>RhoB</t> mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and <t>RhoC</t> protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).
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U73122 PLC rho kinase inhibitor y27632
A . Alignment of potential miR-21 binding sites in the 3′UTR of <t>the</t> <t>RhoB</t> mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and <t>RhoC</t> protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).
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Becton Dickinson rho antibody detecting all rhoa-like species (i.e. rhoa, b and c)
A . Alignment of potential miR-21 binding sites in the 3′UTR of <t>the</t> <t>RhoB</t> mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and <t>RhoC</t> protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).
Rho Antibody Detecting All Rhoa Like Species (I.E. Rhoa, B And C), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech paper n a his rhod
A . Alignment of potential miR-21 binding sites in the 3′UTR of <t>the</t> <t>RhoB</t> mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and <t>RhoC</t> protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).
Paper N A His Rhod, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABclonal Biotechnology a19106
List of primary antibodies
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ABclonal Biotechnology rhoa a15641
List of primary antibodies
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Boster Bio rho a
List of primary antibodies
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Shanghai Korain Biotech Co Ltd human rho-related gtp-binding protein rhob
List of primary antibodies
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Image Search Results


A . Alignment of potential miR-21 binding sites in the 3′UTR of the RhoB mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and RhoC protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).

Journal: PLoS ONE

Article Title: MicroRNA-21 Exhibits Antiangiogenic Function by Targeting RhoB Expression in Endothelial Cells

doi: 10.1371/journal.pone.0016979

Figure Lengend Snippet: A . Alignment of potential miR-21 binding sites in the 3′UTR of the RhoB mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and RhoC protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).

Article Snippet: Blots were blocked for 1 h at room temperature with 8% milk in Tris-buffered saline with 0.1% Tween 20 and probed overnight at 4°C with anti-RhoB, RhoA or RhoC primary antibody (RhoA sc-418; RhoB sc-180, RhoC sc-26480 Santa Cruz Biotechnology) (or for 1 h at room temperature for anti-ERK1,2 M-5670, VCL V-9131, Sigma; Sox7 sc-134024, Santa Cruz Biotechnology; MPRIP ab56164, Abcam and 2 h at 37°C for SPRY1 sc-380048 Santa Cruz Biotechnology).

Techniques: Binding Assay, Transfection, Control, Quantitative RT-PCR, Western Blot, Software, Activity Assay, Fluorescence, Labeling, Staining

List of primary antibodies

Journal: iScience

Article Title: Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate

doi: 10.1016/j.isci.2024.111290

Figure Lengend Snippet: List of primary antibodies

Article Snippet: RhoA , Rabbit, monoclonal , – , 1:1000 , Abclonal, Cat. A19106.

Techniques: