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UTECH Products Inc
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U73122 PLC
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Becton Dickinson
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Proteintech
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ABclonal Biotechnology
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ABclonal Biotechnology
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Boster Bio
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Shanghai Korain Biotech Co Ltd
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Image Search Results
Journal: PLoS ONE
Article Title: MicroRNA-21 Exhibits Antiangiogenic Function by Targeting RhoB Expression in Endothelial Cells
doi: 10.1371/journal.pone.0016979
Figure Lengend Snippet: A . Alignment of potential miR-21 binding sites in the 3′UTR of the RhoB mRNA of different species. B . HUVECs were transfected with a precursor of miR-21 (Pre-miR-21) or with a precursor control (Pre-miR-Ctrl) and with a LNA-21 or with a LNA control (LNA Ctrl). The RhoB mRNA level was analyzed after 48 h by qRT-PCR. C–D . Total protein was extracted from HUVECs 48 h post transfection and RhoA, RhoB and RhoC protein levels were measured by Western blotting. ERK1/2 level was analyzed as an internal control. D . Quantification of ( C ). Quantification was performed using ImageJ software. E–F . Measurement of GTPase activity of RhoB. E . Transfected HUVECs were processed for pull-down assays and Western blot analysis with specific antibody to RhoB. F . Quantification of ( E ). Quantification was performed using ImageJ software. G . Transfected HUVECs were seeded onto gelatin-coated slides and analyzed for fluorescence labeling by phalloidin-FITC (green). Nuclei were visualized by DAPI staining (blue). White arrows show areas with less actin stress fibers. Asterisks represent some elongated HUVECs. Arrowhead indicates stress fibers in the center of the cell. Pictures are representative of three independent experiments. Data are means with the SD. *p<0.05 versus corresponding control; (n = 3).
Article Snippet: Blots were blocked for 1 h at room temperature with 8% milk in Tris-buffered saline with 0.1% Tween 20 and probed overnight at 4°C with anti-RhoB, RhoA or
Techniques: Binding Assay, Transfection, Control, Quantitative RT-PCR, Western Blot, Software, Activity Assay, Fluorescence, Labeling, Staining
Journal: iScience
Article Title: Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate
doi: 10.1016/j.isci.2024.111290
Figure Lengend Snippet: List of primary antibodies
Article Snippet: RhoA , Rabbit, monoclonal , – , 1:1000 ,
Techniques: